Figure 8.

Analysis of the overexpression and deletion of the MexJK efflux pump in the production of different virulence factors regulated by QS. The production of (A) pyocyanin, (B) elastase, (C) rhamnolipids, and (D) pyoverdine was analyzed in supernatants of PAO1, ΔmexL, and ΔmexK strains grown in LB medium for 20 h. For (E) swarming and (F) swimming motility assays, a specific medium was used as described in Methods. For the analysis of (G) biofilm production, a modification of the Calgary device was used. Values that are significantly different by an unpaired two-tail t-test are indicated by asterisks as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.