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. 2022 Jul 15;12(7):652. doi: 10.3390/metabo12070652

Figure 2.

Figure 2

Expression of MTHFD2 and EZH2 depends on mutated KRAS in human AC cell lines. (a) Western blot analysis of EZH2 and MTHFD2 expression in the AC cell lines HCC44, H23, H1993, and HCC78. (b) Cellular survival of HCC44, H23, H1993, and HCC78 cells after 48 h treatment with the KRASG12C inhibitor AMG510 (4 μM). (c) EZH2 and MTHFD2 expression in AC cells after treatment with AMG510 (4 μM) for 48 h. (d) Western blot analysis of EZH2 and MTHFD2 expression in H1993 cells transfected with KrasG12Cvec or KrasWTvec plasmids. (e) Cellular survival of KrasWTvec- and KrasG12Cvec-transfected H1993 cells after 48 h treatment with AMG510 (4 μM). (f) Western blot analysis of indicated proteins in KrasWTvec- or KrasG12Cvec-transfected H1993 cells that were treated as in (e). (*** p < 0.001, **** p < 0.0001).