Table 2.
Top DEGs in the different L. infantum lines after late THP-1 cell infection.
| Gene ID | Description | Leishmania Line | Log2 FC | FDR |
|---|---|---|---|---|
| 1. Transport, uptake, and efflux across cellular membranes | ||||
| LINF_160007200 | Mitochondrial ornithine transporter 1-like protein | LLM2221 | −1.38 | 4.61 × 10−9 |
| LINF_130020800 | Phospholipid-transporting ATPase 1-like protein | LEM3323 | −1.13 | 4.33 × 10−16 |
| LINF_320010400 | LEM3 (ligand-effect modulator 3) family/CDC50 family, putative | LEM5159 | 1.01 | 3.13 × 10−17 |
| LEM2126 | 2.34 | 0.000207 | ||
| LINF_220020300 | Aquaporin, putative | LLM2070 | 1.20 | 1.66 × 10−31 |
| LINF_230007200 | ATP-binding cassette protein subfamily C, member 1, putative | LEM5159 | 1.04 | 3.13 × 10−17 |
| LINF_230007300 | ATP-binding cassette protein subfamily C, member 2, putative | LEM5159 | 1.01 | 4.16 × 10−21 |
| LINF_110018150 | ATP-binding cassette protein subfamily A, member 2, putative | LLM2165 | 1.67 | 4.87 × 10−140 |
| 2. Energy metabolism | ||||
| LINF_230007900 | Argininosuccinate synthase, putative | LEM5159 | 1.79 | 2.69 × 10−45 |
| LEM2126 | 2.12 | 1.61 × 10−49 | ||
| LLM2070 | 1.83 | 1.38 × 10−63 | ||
| LLM2255 | 1.60 | 2.23 × 10−25 | ||
| LLM2165 | 1.37 | 3.22 × 10−32 | ||
| LINF_010010100 | Fatty-acyl-CoA synthetase 2, putative | LEM3323 | 1.43 | 4.06 × 10−33 |
| LEM2126 | 1.26 | 1.59 × 10−21 | ||
| LLM2221 | 1.32 | 4.60 × 10−21 | ||
| LLM2165 | 1.07 | 2.85 × 10−18 | ||
| LINF_230009100 | Cytochrome c oxidase subunit 10, putative | LEM5159 | 1.02 | 4.96 × 10−12 |
| LINF_290032900 | ATP-dependent phosphofructokinase | LLM2070 | 1.44 | 1.14 × 10−49 |
| 3. Redox metabolism and detoxification | ||||
| LINF_270012800 | Amino acid transporter, putative | LEM3323 | 1.00 | 1.55 × 10−20 |
| LEM2126 | 1.90 | 2.50 × 10−85 | ||
| LLM2221 | 1.46 | 3.52 × 10−43 | ||
| LLM2255 | 1.10 | 1.93 × 10−27 | ||
| LLM2165 | 1.75 | 8.01 × 10−94 | ||
| LINF_290017300 | Tryparedoxin-like protein | LLM2070 | 1.30 | 1.48 × 10−18 |
| LINF_150018800 | Tryparedoxin peroxidase | LEM2126 | 1.10 | 2.51 × 10−50 |
| LINF_260013000 | Type II (glutathione peroxidase-like) tryparedoxin peroxidase | LLM2165 | 1.58 | 1.00 × 10−63 |
| LINF_350008900 | Thioredoxin, putative | LLM2070 | 1.21 | 1.94 × 10−15 |
| LINF_260011700 | Thioredoxin, putative | LLM2221 | 1.01 | 6.25 × 10−14 |
| LINF_010006900 | Low-molecular-weight phosphotyrosine protein phosphatase, putative | LEM3323 | 1.10 | 9.28 × 10−19 |
| LINF_180022300 | Gamma-glutamylcysteine synthetase, putative | LEM5159 | 1.03 | 4.98 × 10−10 |
| LEM2126 | 1.65 | 1.81 × 10−40 | ||
| LLM2165 | −1.04 | 1.04 × 10−12 | ||
| LINF_340011100 | Leucine-rich repeat protein, putative | LEM3323 | −1.36 | 3.02 × 10−9 |
| LEM2126 | 1.22 | 3.05 × 10−16 | ||
| LINF_320009800 | Leucine-rich repeat, putative | LEM3323 | 10.25 | 2.69 × 10−104 |
| LEM5159 | 10.18 | 9.34 × 10−108 | ||
| LEM2126 | 10.21 | 8.44 × 10−109 | ||
| LLM2221 | 10.14 | 2.12 × 10−121 | ||
| LLM2070 | 10.08 | 7.91 × 10−108 | ||
| LLM2255 | 10.74 | 4.15 × 10−160 | ||
| LLM2165 | 10.26 | 1.49 × 10−121 | ||
| LINF_050015100 | 3-mercaptopyruvate sulfurtransferase | LEM5159 | −1.25 | 1.82 × 10−9 |
| LEM2126 | −1.24 | 2.38 × 10−10 | ||
| LLM2221 | −1.76 | 9.80 × 10−18 | ||
| LLM2255 | −1.06 | 6.27 × 10−9 | ||
| LINF_220010000 | Acetyltransferase (GNAT) family, putative | LEM3323 | −1.21 | 7.79 × 10−22 |
| LLM2221 | −1.28 | 2.13 × 10−24 | ||
| LINF_330034900 | Metallopeptidase, clan MF, family M17 | LEM3323 | 1.18 | 9.33 × 10−31 |
| LINF_080005000 | Adaptor complex protein (AP) 3 delta subunit 1, putative | LEM3323 | −1.79 | 1.25 × 10−64 |
| LLM2221 | −1.52 | 5.91 × 10−45 | ||
| LLM2255 | 1.07 | 6.32 × 10−33 | ||
| LINF_170016000 | META domain containing protein, putative | LEM2126 | 1.59 | 2.73 × 10−85 |
| LLM2165 | −1.28 | 1.61 × 10−42 | ||
| LINF_230016800 | Alcohol dehydrogenase-zinc-containing-like protein | LEM5159 | 1.21 | 1.63 × 10−22 |
| LINF_290015200 | 5-histidylcysteine sulfoxide synthase, putative | LLM2070 | 1.57 | 6.10 × 10−46 |
| LINF_290007800 | D-lactate dehydrogenase-like protein | LEM2126 | 2.02 | 6.78 × 10−42 |
| LINF_310025400 | Acetylornithine deacetylase-like protein | LLM2255 | 1.06 | 1.11 × 10−6 |
| LLM2165 | 1.29 | 5.34 × 10−10 | ||
| LINF_220017900 | ChaC-like protein, putative | LLM2255 | −1.46 | 1.59 × 10−16 |
| LLM2165 | −1.50 | 6.53 × 10−18 | ||
| LINF_320009700 | Prostaglandin f synthase, putative | LEM3323 | 10.85 | 9.59 × 10−166 |
| LEM5159 | 11.25 | 1.29 × 10−215 | ||
| LEM2126 | 10.69 | 8.55 × 10−167 | ||
| LLM2221 | 10.95 | 6.08 × 10−140 | ||
| LLM2070 | 11.17 | 1.74 × 10−215 | ||
| LLM2255 | 11.50 | 4.43 × 10−219 | ||
| LLM2165 | 11.24 | 1.72 × 10−201 | ||
| 4. Hypothetical | ||||
| LINF_200016760 | Unspecified product | LEM3323 | −5.77 | 6.91 × 10−64 |
| LEM5159 | −4.08 | 6.84 × 10−41 | ||
| LEM2126 | −1.99 | 1.30 × 10−22 | ||
| LLM2221 | −6.16 | 1.47 × 10−64 | ||
| LLM2070 | −2.02 | 1.38 × 10−27 | ||
| LLM2255 | −1.73 | 9.19 × 10−21 | ||
| LLM2165 | −2.11 | 4.98 × 10−28 | ||
Profiling of the differentially expressed genes (DEGs) from L. infantum lines after late-timepoint infection (96 h) of THP-1 cells as described in the Materials and Methods section. The analysis was based on the log2 FC and false discovery rates (FDRs). All the genes presented in this list are statistically significant with an FDR-value ≤ 0.05.