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. 2022 Jun 25;8(7):404. doi: 10.3390/gels8070404

Figure 9.

Figure 9

Effect of HepG2 cell culture in 3D BSAMA cryogels and on 2D substrates on liver-specific gene expression at day 14. HepG2 cells were cultured in 3D and 2D culture systems, and their RNA was extracted for the quantitative real-time PCR analysis of AAT, CYP3A7, G6Pase, HNF4α, HNF6, E-cadherin, N-cadherin, ZO-1, claudin-1, CYP3A4, albumin. The data were normalized to the housekeeping gene GAPDH. (n = 3, *: p < 0.05, **: p < 0.01, ***: p < 0.005, ****: p < 0.001, compared to 2D culture. #: p < 0.05, ##: p < 0.01, ###: p < 0.005, ####: p < 0.001, between the samples at the extremities of the line.) AAT: alpha 1-antitrypsin, G6Pase: glucose 6-phosphatase, HNF: hepatocyte nuclear factors, ZO-1: zonula occludens.