FIG. 2.

Enrichments of wall painting sample H6 monitored by FISH analysis using the specific eubacterium probe EUB338 labeled with the Cy5 reactive fluorescent dye (32). Samples were examined with an epifluorescence microscope (Axioplan; Zeiss) equipped with a 100-W mercury lamp and filter sets for visualization of the fluorescent dyes fluorescein isothiocyanate (green), CY3 (red), and CY5 (far red). For capturing digitized images a slow-scan charge-coupled device camera was used. (A and B) Monitoring of the bacterial growth on M2 (20% NaCl) (A) and MM (10% NaCl) (B) media, using the probe EUB338-Cy5. (C) Halobacillus strain K3-1 isolated from sample H6 growing in MM (10% NaCl) medium, detected by in situ hybridization using the probe EUB338-Cy5.