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. 2022 Jun 28;14(7):1421. doi: 10.3390/v14071421

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of hydrogenated magnolol on MSRV infection steps in GCO cells. (A,C,E,G) illustrate the workflows of the assay design followed in (B,D,F,H), respectively. (B) The infectivity of MSRV was not affected by hydrogenated magnolol. (D) MSRV adhesion was not influenced by hydrogenated magnolol. (F) The replication of MSRV in the 6–8 h period was inhibited by hydrogenated magnolol. (H) The release of MSRV particles in GCO cells was restrained by hydrogenated magnolol. Relative glycoprotein G gene expression was analyzed by qRT-PCR and calculated on the basis of the 2^−ΔΔCt method. The viral titers in the supernatant were calculated using the Karber method at the indicated times. Error bars indicate the SEM. ** p < 0.01.