Table 3.

Analytical sensitivity of Singleplex SADS-CoV PCR assays 2 and 3 by testing serial dilutions of a SADS-CoV cell culture isolate *.

Dilution	Theoretical Titer (TCID50/mL)	SADS-CoV ASSAY 2			SADS-CoV ASSAY 3
		Result 1	Result 2	Result 3	Result 1	Result 2	Result 3
10−2	5 × 105	20.64	20.68	20.69	20.76	20.76	20.69
10−3	5 × 104	24.05	24.03	24.03	24.01	23.97	24.13
10−4	5 × 103	27.51	27.47	27.59	27.44	27.36	27.42
10−5	5 × 102	31.00	30.99	31.23	31.10	31.06	31.24
10−6	5 × 101	34.37	34.25	33.98	34.78	34.10	34.95
10−7	5 × 100	38.45	37.24	38.23	37.45	37.52	36.58
10−8	5 × 10−1	≥40	≥40	≥40	37.26	37.15	≥40
10−9	5 × 10−2	≥40	≥40	≥40	≥40	≥40	≥40

* The work was conducted in Huang’s lab in China.