Table 7.

Repeatability of PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex PCR by testing serial dilutions of in vitro transcribed RNA.

PEDV N-Gene IVT RNA (Genomic Copies per Reaction)	Intra-Assay (3 Replicates for Each Dilution)		Inter-Assay (3 Plates for Each Dilution)
	Mean Ct Value	CV, %	Mean Ct Value	CV, %
8.0 × 106	14.82	1.05	14.90	0.68
8.0 × 105	18.22	0.81	18.21	0.15
8.0 × 104	21.28	0.83	21.16	0.76
8.0 × 103	24.87	0.80	24.88	0.85
8.0 × 102	28.12	0.42	28.31	1.35
8.0 × 101	31.50	0.35	31.51	0.78
8.0 × 100	35.93	1.38	35.73	0.78
	Average intra-assay CV, %	0.81	Average inter-assay CV, %	0.76
PDCoV N-Gene IVT RNA (Genomic Copies per Reaction)	Intra-Assay (3 Replicates for Each Dilution)		Inter-Assay (3 plates for Each Dilution)
	Mean Ct Value	CV, %	Mean Ct Value	CV, %
8.0 × 106	14.82	0.49	15.18	2.09
8.0 × 105	18.24	0.16	18.53	1.45
8.0 × 104	22.31	0.13	22.64	1.28
8.0 × 103	24.58	0.68	24.65	0.26
8.0 × 102	28.52	0.55	28.69	0.51
8.0 × 101	32.67	0.24	33.11	1.16
8.0 × 100	35.90	1.56	36.41	1.37
	Average intra-assay CV, %	0.54	Average inter-assay CV, %	1.16
TGEV S-Gene IVT RNA (Genomic Copies per Reaction)	Intra-Assay (3 Replicates for Each Dilution)		Inter-assay (3 plates for each dilution)
	Mean Ct Value	CV, %	Mean Ct Value	CV, %
8.0 × 106	15.39	0.10	15.43	0.23
8.0 × 105	18.92	0.36	19.09	0.78
8.0 × 104	22.92	0.22	23.01	0.37
8.0 × 103	25.57	0.16	25.59	0.27
8.0 × 102	29.50	0.09	29.85	1.13
8.0 × 101	33.51	0.67	33.77	0.86
8.0 × 100	37.90	3.01	37.67	1.13
	Average intra-assay CV, %	0.66	Average inter-assay CV, %	0.68
SADS-CoV N-Gene IVT RNA (Genomic Copies per Reaction)	Intra-Assay (3 Replicates for Each Dilution)		Inter-Assay (3 Plates for Each Dilution)
	Mean Ct Value	CV, %	Mean Ct Value	CV, %
1.36 × 107	14.50	0.39	14.49	0.13
1.36 × 106	17.81	1.10	17.57	1.37
1.36 × 105	21.77	0.35	21.43	1.41
1.36 × 104	24.76	0.69	24.59	0.68
1.36 × 103	28.57	0.89	28.31	0.88
1.36 × 102	31.92	0.83	32.06	1.09
1.36 × 101	35.30	1.32	35.54	0.90
	Average intra-assay CV, %	0.80	Average inter-assay CV, %	0.92