Table 9.
Diagnostic performance of PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex PCR in comparison with the reference PEDV/TGEV/PDCoV PCR on clinical samples *.
| Reference PCR—PEDV | ||||
|---|---|---|---|---|
| Positive | Negative | Total | ||
| 5-plex PCR—PEDV | Positive | 95 | 6 | 101 |
| Negative | 1 | 117 | 118 | |
| Total | 96 | 123 | 219 | |
| Sensitivity 98.96%; specificity 95.12%; agreement 96.80% | ||||
| Reference PCR—PDCoV | ||||
| Positive | Negative | Total | ||
| 5-plex PCR—PDCoV | Positive | 82 | 3 | 85 |
| Negative | 0 | 134 | 134 | |
| Total | 82 | 137 | 219 | |
| Sensitivity 100%; specificity 97.81%; agreement 98.63% | ||||
| Reference PCR—TGEV | ||||
| Positive | Negative | Total | ||
| 5-plex PCR—TGEV | Positive | 12 | 0 | 12 |
| Negative | 0 | 207 | 207 | |
| Total | 12 | 207 | 219 | |
| Sensitivity 100%; specificity 100%; agreement 100% | ||||
* Ct < 37 was considered positive for the PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex and Ct < 36 was considered positive for the reference PEDV/TGEV/PDCoV PCR.