Table 10.
Discrepancies on clinical samples between the PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex PCR and the reference PEDV/TGEV/PDCoV PCR *.
| Sample ID | Specimen | Reference PCR | 5-Plex PCR | |||||
|---|---|---|---|---|---|---|---|---|
| PEDV | PDCoV | TGEV | PEDV | PDCoV | TGEV | SADS-CoV | ||
| Sample_#23 | Fecal swab | 36.4 | ≥40 | ≥40 | 36.6 | ≥40 | ≥40 | ≥40 |
| Sample_#24 | Fecal swab | 36.4 | ≥40 | ≥40 | 35.1 | ≥40 | ≥40 | ≥40 |
| Sample_#27 | Fecal swab | 39.4 | ≥40 | ≥40 | 36.4 | ≥40 | ≥40 | ≥40 |
| Sample_#75 | Feces | 36.3 | 34.6 | ≥40 | 35.4 | 31.4 | ≥40 | ≥40 |
| Sample_#138 | Oral fluid | 35.0 | ≥40 | ≥40 | ≥40 | ≥40 | ≥40 | ≥40 |
| Sample_#144 | Oral fluid | 38.5 | ≥40 | ≥40 | 35.6 | ≥40 | ≥40 | ≥40 |
| Sample_#154 | Oral fluid | ≥40 | 28.6 | ≥40 | 35.8 | 27.4 | ≥40 | ≥40 |
| Sample_#113 | Oral fluid | 21.5 | 37.0 | ≥40 | 20.2 | 33.5 | ≥40 | ≥40 |
| Sample_#115 | Oral fluid | 24.6 | 36.8 | ≥40 | 23.8 | 36.4 | ≥40 | ≥40 |
| Sample_#145 | Oral fluid | 39.0 | 37.7 | ≥40 | 39.2 | 36.0 | ≥40 | ≥40 |
* Ct < 37 was considered positive for the PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex and Ct < 36 was considered positive for the reference PEDV/TGEV/PDCoV PCR.