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Tunicamycin inhibits epithelial-to-mesenchymal transition in human CRC cell lines. SW480 and HCT116 cells were exposed to tunicamycin (0.1 μg/mL, 0.5 μg/mL). (A) Wound healing test revealed that tunicamycin inhibited HCT116 and SW480 cell migration. (B–D) SW480 and HCT116 cells were exposed to tunicamycin (0.1 μg/mL, 0.5 μg/mL) for 6 h, and then analyzed for protein and mRNA expression. qRT-PCR and Western blotting results showed the expression of proteins and mRNA involved in EMT.
