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. 2022 Jul 7;11(14):2141. doi: 10.3390/cells11142141

Figure 4.

Figure 4

The effect of the number of lox71 sites on the transgene expression. Left panel—flow cytometry analysis of HEK293T cells transfected with the pVax1-lox71-C-GFP with one, three or six lox71 sites or control plasmids 48 h after the transfection. Right panel—fluorescent microscopy of transfected cells 48 h after the transfection. The number of lox71 sites is indicated within the construct name. Inductor: transfection by dCreΔ331-VP4 gene for lox71 system and doxycycline for TRE3G promoter. *, The percentage of bright HEK293T cells among all GFP-positive cells; **, p < 0.05 compared to GFP fluorescence intensity of cells transfected with the appropriate plasmids without induction, n ≥ 30,000. Inductors: doxycycline for TetON 3G-GFP and dCreΔ331-VP4 for lox711/3/6-containing plasmids. In the diagram, data are presented as the median (25%; 75%).