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. 2022 Jul 7;11(14):2141. doi: 10.3390/cells11142141

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Efficiency of transgene expression in the CreVP64/lox71 system. (A) Flow cytometry of cells transfected with constructs encoding PKA-spark. *, The percentage of bright HEK293T cells among all GFP-positive cells. (B) Fluorescence microscopy of cells transfected with constructs encoding PKA-spark. (C,F) Fold enhancement (to “before induction” level) of PKA-spark (C) and BDNF (F) mRNAs in HEK293T cells 48 h after induction. (D) PKA-spark sensor fluorescence intensity of cells transfected with pVax1-lox71_3/6-C-PKA-spark with or without induction. **, p < 0.05, n ≥ 30,000. Data are presented as the median (25%; 75%). (E) The secretion of human BDNF by transfected cells measured by ELISA 48 h after induction.