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Results of TP53 gene sequencing within the range of genome editing in HEK293T cells after induction of Cas9 programmable nuclease expression. (A) An alignment of TP53 allele sequences within the range of genome editing; the blue line labels the gRNA binding region, the red frame marks the PAM sequence and the red triangle marks the Cas9 cleavage region. (B) The result from the analysis of the sequencing data in the TIDE program.
