TABLE 2.
Variation in rpoS sequences of E. coli O157:H7, O124, and K-12 strains
| Strain | Serotype | Base (amino acid) at position:
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −306 | −294 | −183 | −162 | −94/−87 | −96/−98a | 55–57 | 61–63 | 214–216 | 382–384 | 387a | 423 | 442–444 | 543a | 606 | 714 | 819a | 910–91 | ||
| DH1 | —(K-12) | C | T | T | G | CTG (Leu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ATC (IIe) | C | C | TGG (Trp) | C | G | G | G | CAG (Glu) | |
| EDL933 | O157:H7 | T | C | G | A | GAG (Glu) | GAC (Asp) | CGA (Arg) | ACG (Thr) | ATC (IIe) | C | C | TGG (Trp) | A | G | G | A | CAG (Glu) | |
| C9490 | O157:H7 | T | C | G | A | GAG (Glu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ATC (IIe) | T | C | TGG (Trp) | A | G | G | A | CAG (Glu) | |
| 30-2C4 | O157:H7 | T | C | G | A | GAG (Glu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ATC (IIe) | T | C | TGG (Trp) | A | G | G | A | CAG (Glu) | |
| 12079 | O157:H7 | T | C | G | A | GAG (Glu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ACC (Thr) | T | C | TGG (Trp) | A | G | G | A | CAG (Glu) | |
| W2-2b | O157:H7 | T | C | G | A | (TAAACCCG) | GAG (Glu) | GAG (Glu) | GGA (Gly) | CCG (Pro) | ATC (IIe) | T | C | TGG (Trp) | A | G | G | A | CAG (Glu) |
| H1071 | O157:H7 | T | C | G | A | GAG (Glu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ATC (IIe) | T | C | TAG | C | G | G | A | CAG (Glu) | |
| 8003 | O124 | C | C | G | A | GAG (Glu) | GAG (Glu) | GGA (Gly) | ACG (Thr) | ATC (IIe) | C | T | TGG (Trp) | C | A | A | A | TAG | |
a
Mutations already noted by and in agreement with Ferreira et al. (5).
b
The transcriptional start site of rpoS in W2-2 differs from that of the other strains by 8 nt resulting from the insertion of a duplicated region at nt −94 to −87. Therefore, in strain W2-2 the positions of the bases before the start site are displaced compared with the other strains, with nt −306, −294, −183 and −162 in the other strains being equivalent to nt −314, −302, −191, and −170, respectively, in strain W2-2.