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. 2022 Jul 14;23(14):7755. doi: 10.3390/ijms23147755

Figure 4.

Figure 4

Relative expression of anthocyanin synthesis-related genes in ‘Daihong’ tissue culture seedlings grown in mediums with 4 and 0 mM NO3−. (A) Relative expression of structural genes involved in anthocyanin synthesis (a: MdPAL, b: MdCHS, c: MdCHI, d: MdF3H, e: MdDFR, f: MdANS, g: MdANR, h: MdUFGT). (B) Relative expression of regulatory genes related to anthocyanin synthesis (a: MdMYB10, b: MdbHLH33, c: MdbHLH3, d: MdWD40). The relative level of anthocyanin synthesis-related genes in ‘Dahong’ tissue culture seedlings at 0 h was set to 1. The bars represent means ± SD (n = 3). Asterisks indicate statistically significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).