Table 1.
Table of antibodies, vendor catalog number, dilution factor by application, and lot number.
| Antibody | Vendor/Catalog Number | Application/Dilution | Lot Number |
|---|---|---|---|
| Claudin-5 Mouse mAb (4C3C2) | Invitrogen | WB: 1:500 | WD |
| 35-2500 | ICC: 1:50 | 327318 | |
| VE-Cadherin Rabbit pAb (CD144) | Invitrogen | WB: 1:500 | UF |
| 36-1900 | ICC: 1:200 | 287723 | |
| Zona Occludens-1 Mouse mAb (ZO1-1A12) | Invitrogen | ICC: 1:200 | WG |
| 33-9100 | 329571 | ||
| α-Tubulin (DM1A) Mouse mAb (DM1A) | Cell Signaling Technology | WB: 1:10,000 | 16 |
| 3873 | |||
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 |
Invitrogen A-21206 |
ICC: 1:10,000 | 2156521 |
| Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 |
Invitrogen A-10037 |
ICC: 1:10,000 | 2156521 |
| IRDye 800CW Donkey anti-Rabbit IgG Secondary Antibody | Li-Cor 926-32213 |
WB: 1:10,000 | D10518-05 |
| IRDye 680RD Donkey anti-Rabbit IgG Secondary Antibody | Li-Cor 926-68072 |
WB: 1:10,000 | D10728-15 |
| Alexa FluorTM 488 Phalloidin IR Dye 800CW Donkey anti-Rabbit IgG Secondary Antibody | Invitrogen A12379 |
WB: 1:10,000 ICC 1:40 |
D10518-052219253 |