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. 2022 Jul 9;23(14):7612. doi: 10.3390/ijms23147612

Figure 1.

Figure 1

Expression of recombinant TP84_28 gene in three different E. coli cells. (a) Growth curves of bacterial clones, carrying recombinant pTP84_28 plasmids containing the TP84_28 gene in various E. coli expression strains: BL21(DE3), BL21(DE3) (pLysS), and ARCTICA. (b) E. coli BL21(DE3) (pTP84_28), grown on solid LB medium. Only this strain exhibited the characteristic rim around the bacterial colony, indicating interference of the cloned TP84_28 gene with the bacteria’s growth rate.