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Determination of the thermal stability of recombinant TP84_28 endolysin. (a) TRA conducted on the pre-incubated enzyme and quantitative spectrophotometric measurements taken using a G. stearothermophilus strain 10 cell suspension; the dots represent the activity level of TP84_28 relative to the enzyme pre-incubation temperature (b) CD analysis; (c) DSC analysis; the heat-capacity curve of TP84_28 (black line) and the fit to the non-two-state model (red line).
