Figure 6.

Effect of PTX-loaded NMeds on cell health of C6 glioblastoma cells. (a) Quantification of apoptosis using FITC-Annexin V. The percent of Annexin V positive cells of total cells is shown as average from n = 3 OFV. C6 cells were treated for 3 h with empty non-targeted and M08-targeted NMeds, and non-targeted and M08-targeted NMeds delivering the equivalent of 10, 50, 100, and 500 nM PTX. Free PTX at the same concentrations was used as controls. (b) Representative images showing all cells. The visualisation was achieved by labelling the nuclei with Hoechst staining and labelling apoptotic cells with Annexin V-FITC. (c,d) Cell impedance measurement after treatment with empty non-targeted and M08-targeted NMeds, as well as their PTX loaded counterparts (the equivalent of 500 nM PTX) and compared with500 nM free PTX as a control treatment. Before treatment, cells were grown for 12.5 h (t0₁—750 min). n = 3 per treatment. Cell death was evaluated as a decrease in impedance and shown as % of t0. (d) Cell death analysis at the 14 h endpoint of the treatment shown in (c). One-way ANOVA analysis and Tukey’s Post hoc tests were performed, * p < 0.05, ** p < 0.01, *** p < 0.001.