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. 2022 Jul 21;14(7):1511. doi: 10.3390/pharmaceutics14071511

Figure 2.

Figure 2

Figure 2

The effects of tylophorine-based dbq33b and ciclesonide on IκBα protein levels in HCoV-OC43-infected fetal lung fibroblast MRC-5 cells. (A) Effects of tylophorine-based dbq33b on the IκBα protein levels in HCoV-OC43-infected MRC-5 cells over a period of 30 h. (B) Ciclesonide treatment increased IκBα protein levels in HCoV-OC43-infected MRC-5 cells over a period of 30 h. (C) Ciclesonide decreased transcriptional levels of IκBα at 6 h.p.i. (a) and 30 h.p.i. (b). Uninfected and HCoV-OC43-infected (MOI: 0.05) MRC-5 cells treated with tylophorine-based dbq33b or ciclesonide (5 μM pretreatment for 1 h) were harvested at the indicated h.p.i. The resulting lysates were subjected to western analyses with the antibodies indicated (A,B). For transcriptional analyses, the uninfected and HCoV-OC43-infected (MOI: 0.05) MRC-5 cells treated with different concentrations of ciclesonide (0, 1.25, 2.5, 5 μM pretreatment for 1 h) were, respectively, harvested at 6 h.p.i. and 30 h.p.i. The TRIzol™ Reagent (Invitrogen, 15596018) was used to extract total RNA from the resultant cell lysates prior to performing RT-PCR using the specific primer pairs for the genes indicated. (C) Results shown are representative of three independent experiments and AVE ± S.D. from three independent experiments. (* p < 0.05, ** p < 0.01 for student’s t-test).