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. 2022 Jul 21;14(7):1511. doi: 10.3390/pharmaceutics14071511

Figure 3.

Figure 3

The effects of combined treatment with ciclesonide and tylophorine-based dbq33bon on the p65 nuclear translocation/NF-κB activation induced by HCoV-OC43 infection in MRC-5 cells. (A) Scheme for the sequence in which the HCoV-OC43 infection and compound treatments were performed. (B) The effect of ciclesonide on p65 translocation into nuclei induced by HCoV-OC43 infection in MRC-5 cells. (C) The effects of tylophorine-based dbq33b alone or in combination with ciclesonide on p65 translocation into nuclei induced by HCoV-OC43 infection in MRC-5 cells. After fixation, cells were treated with CF®488A goat anti-rabbit IgG for anti-p65 (green) and their nuclei visualized by staining with Hoechst (blue). Images were acquired using an ImageXpress Micro XLS Wide field High-Content Screening System under “autofocus” function and the best z-offset. Results shown are representative of three independent experiments and AVE ± S.D. from three independent experiments. (** p < 0.01, *** p < 0.001 for student’s t-test; # p < 0.05, ## p < 0.01, ### p < 0.001 for two-way ANOVA test). (Large images for (B,C) were provided in “Supplemental Data”).