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. 2022 Jun 29;14(7):1381. doi: 10.3390/pharmaceutics14071381

Figure 1.

Figure 1

Construction and schematic representation of anti-EGFR/PD-L1 BsAbs: (A) Dual variable domain immunoglobulin (DVD-Ig) symmetric tetravalent format of anti-EGFR/PD-L1 BsAb in which single-chain variable fragments of ATE and Cetux are connected to the human IgG Fc portion. (B) Knob-in-hole (KIH) asymmetric bivalent format of anti-EGFR/PD-L1 BsAb in which two half-heavy chains containing “knob” mutations (T350V_L351Y_F405A_Y407V) and “hole” mutations (T366L_K392l_T394W) were generated. scFv ATE (light and dark red); scFv Cetux (light and dark blue); human Fc-IgG1 (grey). (C) SDS-PAGE analysis showing the heavy and light chains of DVD-Ig and KIH formats of purified anti-EGFR/PD-L1 BsAbs, ATE and Cetux under non-reducing and reducing conditions.