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PD-1/PD-L1-blocking activity of anti-EGFR/PD-L1 BsAbs was determined by PD-1/PD-L1 blockade cell-based assay (Promega) to assess the ability of the anti-EGFR/PD-L1 BsAbs to block PD-1/PD-L1 engagement compared to parental mAbs. This experiment has been performed in biological triplicates, and data are representative of two or more independent experiments.
