Figure 5.

T cell-mediated killing assay was performed in MDA-MB-231 and BT-20 cells, in which calcein AM-labeled cancer cells were co-cultured with activated T cells, then treated with anti-EGFR/PD-L1 BsAbs, parentals mAbs or left untreated for 24 h. After 24 h of incubation, calcein AM-labeled live cells were counted by Celigo Imaging system. T cell-mediated killing assay was performed in triplicates, and data are representative of two or more independent experiments. The differences between two groups were considered statistically significant when p < 0.05 (*, p < 0.05; **, p < 0.01).