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. 2022 Jun 29;14(7):1381. doi: 10.3390/pharmaceutics14071381

Figure 8.

Figure 8

Anti-EGFR/PD-L1 BsAbs inhibited ligand-induced signaling in TNBC cells. MDA-MB-231 (A), BT-20 (B) and MDA-MB-468 (C) cells were subjected to serum starvation overnight and then pre-treated with anti-EGFR/PD-L1 BsAbs and parental mAbs. After antibody treatment, cells were exposed to EGF for 30 min at a concentration of 50 ng/mL. After treatments, cells were lysed in lysis buffer to prepare whole-cell lysate (WCL), and samples were subjected to western blotting to determine the phosphorylation levels and total protein levels of indicated signaling molecules. Actin expression was performed to measure equal loading. Unprocessed western blots images of (A–C) are provided in Supplementary Figure S2.