Table 1.

List of discovered metabolites of RAD140 in human urine by means of LC-HRMS/MS.

Analyte	Transformation	Molecular Formular	RT [min]	Polarity	Precursor Ions m/z	Product Ions m/z	NCE [%]	Ref.
RAD140	-	C18H11ClN5O−	6.84	−	348.0658	321.0549 127.0302 175.0068	20	[11]
		C20H17ClN5O2+		+	394.1065	223.0633 205.0527 172.0505	30	[12]
M1	hydrolysis hydroxylation sulfation	C8H6ClN2O4S−	3.54	−	260.9742	181.0174 260.9742	25	[12]
M2a	glucuronidation hydroxylation	C26H23ClN5O9−	4.59	−	584.1190	193.0354 540.0928 364.0607	30
M2b	glucuronidation hydroxylation		4.79	−		193.0354 390.0763 170.0360	30
M3	hydrolysis hydroxylation	C8H6ClN2O−	4.98	−	181.0174	145.0407 118.0298	50	[12]
M4	glucuronidation	C26H23ClN5O8−	5.42	−	568.1241	193.0354 113.0244 374.0814	30	[12]
M5	hydrolysis	C8H8ClN2+	5.49	+	167.0371	167.0371 131.0604 104.0495	50	[12]
M6a	hydroxylation	C18H11ClN5O2−	5.76	−	364.0607	170.0360 193.0174 145.0407	30	[11] 1
M6b	hydroxylation		5.91	−		170.0360 193.0174 145.0407	30	[11] 1
M6c	hydroxylation		6.59	−		180.0096 193.0174 145.0407	30	[11] 1
M7	sulfation	C20H15ClN5O5S−	6.25	−	472.0488	348.0658 96.9601	30
S-24	(ISTD 2)	C18H13O3N2F4−	7.45	−	381.0868	241.0594	30	[20]

¹ Only two hydroxy metabolites were reported by Sobolevsky et al. ² Internal standard.