Table 2.

Method characterization results of the LC-HRMS/MS method for the detection of RAD140.

Intra-day imprecision	15.0%	at 2 ng/mL	n = 10
Intra-day imprecision	12.8%	at 1 ng/mL	n = 10
Intra-day imprecision	20.0%	at 0.2 ng/mL	n = 10
Inter-day imprecision	18.5%	at 2 ng/mL	n = 30
Inter-day imprecision	14.9%	at 1 ng/mL	n = 30
Inter-day imprecision	23.4%	at 0.2 ng/mL	n = 30
LOD	9	pg/mL	n = 10
Selectivity	yes		n = 10
LOI	120	pg/mL	n = 10
Recovery	20.6–70.2%	at 1 ng/mL	n = 10
Matrix effects	94.2–122.1%	at 1 ng/mL	n = 10
Extract stability (7 days, 4 °C)	55.9–93.4%	at 1 ng/mL	n = 10
Carryover	0.0%	at 8 ng/mL	n = 1
Robustness	12.4%		n = 10
STDev retention times	0.05%		n = 10
Linearity (R2)	0.9918–0.9990		n = 6