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. 2022 Jul 3;12(7):687. doi: 10.3390/membranes12070687

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of pCECs under static and flow conditions. (a,b) Calcein staining of viable pCECs (green) after 24 h of static (a) or dynamic (15 mL/min flow) (b) conditions; (c) qRT-PCR analysis of flow-exposed pCECs regarding inflammatory activation (E-Selectin and ICAM), shear stress response (KLF 2), thrombogenic state (TM and TF) and matrix synthesis (Col 4 A1). Gene expression levels of the flow-exposed group were compared to the static control groups using an unpaired t-test. For abbreviations of gene names, see Table 2. Results are given as mean with SD (n = 3) (* p < 0.05; ** p < 0.01; ns = no significance).