TABLE 3.
Function of identified circRNAs in other tissues and disease models
| CircRNA | Disease | Function | Cell and tissue type | Study method | PMID |
|---|---|---|---|---|---|
| hsa_circ_0007364 (circPTP4A2) | Cervical cancer |
Upregulated in cervical cancer Promote cervical cancer progression via miR‐101‐5p/MAT2A axis Promote the proliferation and invasion of cervical cancer cell lines Promote cervical cancer growth in vivo |
Cervical cancer and adjacent nontumor tissues Cervical cancer cell lines (HeLa, CaSki, SiHa, C‐33A, C‐4I, SW756) Normal human cervical epithelial cell line (End1/E6E7) |
GEO microarray datasets (GSE113696, GSE102686), RT‐qPCR, Luciferase reporter assay, RIP, cell proliferation assay, cell invasion assay, tumor xenograft in vivo | 33138667 a |
| hsa_circ_0007364 (circPTP4A2) | Glioma | Downregulated in glioma | Glioma and normal brain tissues | GEO microarray dataset (GSE86202) | 31895689 |
| hsa_circ_0007364 (circPTP4A2) | Diabetic kidney disease | Upregulated in human renal proximal tubular epithelial cell line in response to glucose stress | Immortalized human renal proximal tubular epithelial cell line (HK‐2) | Microarray analysis, RT‐qPCR | 32115515 |
| hsa_circ_0005777 (circARHGEF28) | Bladder cancer |
Downregulated in bladder cancer Promote bladder cancer progression through miR‐1305/Tgf‐β2/smad3 pathway targeting EMT Accelerate bladder cancer cells’ progression, including cell viability, proliferation, invasion, migration, and wound healing potential of bladder cancer cell lines |
Bladder cancer and adjacent nontumor tissues Bladder cancer cell lines (5637, UM‐UC‐3) |
RNA‐sequencing, RT‐qPCR, FISH, Dual‐luciferase reporter assay, RNA pulldown assay, cell viability assay, cell proliferation assay, cell migration and matrigel invasion assay, wound healing assay, Western blot, tumor subcutaneous mice model | 32019579 a |
| hsa_circ_0005777 (circARHGEF28) | Gastric cancer | Downregulated in gastric cancer | Gastric cancer and adjacent nontumor tissues | GEO microarray datasets (GSE78092, GSE83521, GSE93541, and GSE100170) | 31346318 |
| hsa_circ_0005777 (circARHGEF28) | Non‐small cell lung cancer |
Upregulated in non‐small‐cell lung cancer Promote non‐small‐cell lung cancer progression via miR‐671‐5p/FOXM1 axis CircRNA expression associated with tumor size, nodule metastasis, and cancer staging |
Non‐small‐cell lung cancer and adjacent nontumor tissues Immortalized lung epithelial cell line (16HBE) Non‐small‐cell lung cancer cell lines (A549, H460, HCC827) |
RT‐qPCR, Western blot, RIP, FISH, RNA pulldown assay, Luciferase reporter assay, cell proliferation assay, cell migration assay | 34291441 |
| hsa_circ_0005777 (circARHGEF28) | Periodontitis | Upregulated in periodontitis | Human gingival tissues | RNA‐sequencing | 31021476 |
| hsa_circ_0067582 (circRASA2) | Gastric cancer |
Downregulated in gastric cancer Associated with gastric cancer patients’ tissue CEA level positively Biomarker for gastric cancer diagnosis |
Gastric cancer and adjacent non‐tumor tissues Healthy gastric mucosa, gastritis mucosa, and gastric intestinal metaplasia tissues |
RT‐qPCR | 31328820 a |
| hsa_circ_0067582 (circRASA2) | Gastric cancer |
Downregulated in gastric cancer Associated with tumor diameter positively and CA19‐9 level negatively Biomarker for gastric cancer diagnosis and prognosis evaluation |
Gastric cancer and adjacent nontumor tissues | RT‐qPCR | 31721300 a |
Abbreviations: CA19‐9, carbohydrate antigen 19‐9; CEA, carcinoembryonic antigen; EMT, epithelial‐mesenchymal transition; FISH, florescent in situ hybridization; GEO, Gene Expression Omnibus; RIP, RNA immunoprecipitation.
CircRNA‐disease associations predicted by online CircR2Disease v2.0 database.