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. 2022 May 5;45(7):2019–2036. doi: 10.1111/pce.14337

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© 2022 State of New South Wales. Plant, Cell & Environment published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Validation of the genetic linkage between Δ¹³C and molecular markers in an F₂ validation population derived from the BC1329/BC9102, grown under non‐stress conditions (Experiment 4). The frequency distribution of Δ¹³C (‰) among 733 F₂ lines (a). Genetic correlations between Δ¹³C, DTF, LWC, and SLW from bivariate analyses are shown (b). The genetic position of flanking markers for Δ¹³C QTL on A09 (c) and C09 (d) and their corresponding physical positions (e, f) in the DH population and physical map positions of DArTseq markers on A09 and C09 chromosomes in an F₂ population. (g, h) Comparative physical locations of markers are shown with lines (in colour). For clarity, physical positions are given in 1/10th fraction of Mb. DH, doubled haploid; DTF, days to flower; LWC, leaf‐water content; QTL, quantitative trait loci; SLW, specific leaf weight; Δ¹³C, carbon isotope discrimination.