Table 1.
Description of phenotyping experiments conducted to uncover the genetic basis of carbon isotope discrimination (Δ13C) and water‐use efficiency‐related traits in canola (B. napus L). The plant development stage at which traits were measured is detailed (Method S1)
| Experiment | Research question | Material | Phenotyping environment | Traits measured |
|---|---|---|---|---|
| 1 | Genetic basis of Δ13C variation | 223 DH lines from BC1329/BC9102, plus parental lines | Field (2017) |
Δ13C Days to flower (DTF) Plant height (PH) Seed yield (SY) |
| 2 | 223 DH lines from BC1329/BC9102, plus parental lines and commercial cultivars | Field (2018) |
Δ13C Normalised difference in the vegetative index (NDVI) DTF PH SY |
|
| 3 | 217 Doubled haploid lines from BC1329/BC9102, plus parental lines | Pots under rain‐out shelter (2017) |
Δ13C DTF PH |
|
| 4 | 733 F2 lines derived from BC1329/BC9102, plus parental lines | Pots in glasshouse (2020) under wet (field capacity) conditions |
Δ13C DTF Specific leaf weight (SLW) Leaf‐water content (LWC) |
|
| 5 | Relationship between physiological (intrinsic water‐use efficiency, Δ13C) and agronomic water‐use efficiency (canola productivity) related traits | Selected 70 DH lines representing extreme (low and high Δ13C values) plus parental lines under wet and dry conditions | Rain‐out shelter (2019) |
Δ13C DTF PH SY Photosynthesis (A) Stomatal conductance (g sw) Intrinsic water‐use efficiency (iWUE) SLW LWC |
| 6 | Identify differentially expressed genes underlying QTL for Δ13C variation | Parental lines of DH population (BC1329 and BC9102) | Pots in glasshouse (2020) under wet and dry conditions | None |
Note: The plant development stage at which traits were measured is detailed in Supporting Information: Method S1.
Abbreviations: DH, doubled haploid; QTL, quantitative trait loci.