A)
Actinomadura sp. RB99 colony (BC) inhibiting growth of garden weed Pseudoxylaria sp. X802 (FC) after 14 d grown on PDA and formation of a zone of inhibition (ZI). B) GNPS based molecular network clusters of target molecular ion features of RB99 co‐cultivated with Pseudoxylaria sp. X802 (14 d, PDA, red nodes represent bacterial colony, blue nodes represent zone of inhibition (ZI). C) GNPS networking features from cultivation on different media (red, ISP2 medium; green, ISP5 sea salt medium; blue, ISP5 medium). D‐E) Extracted ion chromatograms of target molecular ion features when D) RB99 was co‐cultured with Pseudoxylaria sp. X802 and E) RB99 was cultured on different growth media (ISP2, ISP5 sea salt, ISP5), target molecular ion features: m/z 624.2977 (Sum formula C27H42N7O10
+, calcd., 624.2988, all EICs are displayed in 5 ppm range).