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A) Comparison of biosynthetic gene clusters based on amino acid identity (amc (amychelin), rene (madurastatins) and mad (madurastatins)). B) Proposed biosynthetic pathway for the formation of madurastatins (blue: proposed structures obtained via a functional Cyc1 domain; abbreviations: T, thiolation domain; Cyc, Heterocyclization domain; A, adenylation domain; LCL Condensation domain; DCL epimerization and condensation domain).
