FIGURE 4.

RT‐qPCR analysis of CB2r transcript (A) and protein (B) levels measured by western blot following the CSE, CBD, and CAR (1 μg/ml) treatments in unstimulated and LPS‐stimulated BV‐2 cells (250 ng/ml). (C) Immunoblot represents protein levels of CB2r and the endogenous target vinculin. Each column represents mean ± SEM. Data were analyzed by one‐way analysis of variance followed by Tukey: **p < .01, ***p < .0001, versus CTRL group; °p < .05, °°p < .01, versus LPS (n = 6 for mRNA, n = 4 for protein). CAR, β‐caryophyllene; CBD, cannabidiol; CSE, Cannabis sativa extract; LPS, lipopolysaccharide; SEM, standard error of the mean