Figure 4.

Failure of BVRA nuclear translocation led to hypoinduction of HO-1 in senescent LECs under oxidative stress. (a) After being transfected with BVRA siRNA or NC siRNA, the young and senescent LECs were exposed to 200 μM H₂O₂ for 1 h. The relative protein expressions of HO-1 were analyzed by western blotting. (b) The young and senescent LECs were exposed to 200 μM H₂O₂ for 4 h. Cells were immunostained with antibody against BVRA (red) and DAPI (blue). (c) The relative protein expressions of BVRA in the nucleus and cytoplasm were detected by western blot. Data are shown as mean ± SEM, n = 3, one-way ANOVA, ^∗∗P < 0.01 and ^∗∗∗P < 0.001, compared with young LECs. ^#P < 0.05, compared with senescent LECs. ^ψP < 0.05, compared with H₂O₂-treated young LECs. Bar 100 μm.