Figure 5.

Failure of Nrf2 nuclear localization in senescent LECs. (a) The young and senescent LECs were transfected with BVRA siRNA or NC siRNA, followed by 200 μM H₂O₂ exposure for 1 h. The effect of BVRA knockdown on the relative expression of phosphorylated-ERK1/2 and Nrf2 was assessed by western blot. (b) The young and senescent LECs were exposed to 200 μM H₂O₂ for 4 h. The binding capacity of BVRA and ERK2 in LECs was determined by CoIP assay. Data are shown as mean ± SEM, n = 3, one-way ANOVA, ^∗∗P < 0.01 and ^∗∗∗P < 0.001, compared with young LECs.