Skip to main content
. 2022 Jul 19;2022:2748962. doi: 10.1155/2022/2748962

Figure 2.

Figure 2

Identification of anti-BjussuMP-II VHHs. (a) Monitoring of the camelid immune response after administration of BjussuMP-II. Results obtained in an ELISA assay, demonstrating the absorbances obtained in different dilutions of the serum collected on the following days: 0 (∗), 14 (■), 21 (▲), and 28 (•). (b) Analysis of VHHs against the toxin BjussuMP-II after biopanning. The cut-off line corresponds to the average of the cut-off values calculated for each of the 47 clones. (c) Multiple alignments among clones selected against BjussuMP-II, demonstrating CDRs and FRs. Letters highlighted in red represent the cysteines present in all clones. The gray shaded areas represent the CDRs. Amino acids underlined in the FR2 region represent the characteristic amino acids of VHHs. (∗) represents identical amino acids; (:) represents strongly similar amino acids; (.) represents weakly similar amino acids.