HS3ST1 promotes the development of NSCLC by regulating SPOP/FADD/NF-κB pathway. (a) The effects of SPOP overexpression, FADD knockdown, and NF-κB knockdown on the viability of A549 cells. (b) The effects of SPOP overexpression, FADD knockdown, and NF-κB knockdown on the apoptosis of A549 cells. (c) The effects of SPOP overexpression, FADD knockdown, and NF-κB knockdown on the cell cycle distribution of A549 cells. (d) The effects of SPOP overexpression, FADD knockdown, and NF-κB knockdown on the wound healing rate of A549 cells. (e) The effects of SPOP overexpression, FADD knockdown, and NF-κB knockdown on the Transwell cell counts of A549 cells. ∗∗P < 0.01 between two groups, N = 12.