Table 2.

Primer sequences used for methylation-specific PCR

Gene name	Primer sequence (5’ → 3’) sense	Primer sequence (5’ → 3’) antisense	TM (°C)	Product size
HLA-C Methylated	GATCGGAGAGAGTTTTAGTCG	GCTAAATAATCTAAACCGCGA	53	240
HLA-C Unmethylated	GATTGGAGAGAGTTTTAGTTG	AACTTACACTAAATAATCTAAACCACAA	51	260