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. 2022 Jul 26;13:4334. doi: 10.1038/s41467-022-32092-8

Fig. 1. Vβ-specific CAR-T cells specifically eliminated target-positive primary T cells.

Fig. 1

a–c The expression levels of the indicated TCR Vβ subtypes (a, c) or total TCRαβ (b) in untransduced T cells and Vβ8-, Vβ13-, or Vβ5-specific CAR-T cells were analyzed by flow cytometry (a, b) or real-time PCR (n = 3 biologically independent samples/group) (c). d, e Untransduced T cells or Vβ8-CAR-T cells were cocultured with nonfractional normal T cells in triplicate wells at a CAR-T:normal T cell ratio of 10:1 for 24 h (n = 3 biologically independent samples/group). In the remaining Vβ8+ cells, cytotoxicity was determined by flow cytometry (d), and the indicated cytokines in the supernatants were analyzed by performing CBA assays (e). The data shown represent the mean ± standard error of the mean (SEM). Statistical significance was determined by two-sided unpaired t-tests. The normality of the data was confirmed by the Shapiro–Wilk test. Representative results of one of three replicate experiments are shown in a–e.