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. 2022 Jul 26;13:4334. doi: 10.1038/s41467-022-32092-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Untransduced T cells or Vβ8-CAR-T cells were cocultured with Lenti-X 293-Vβ8^- or Lenti-X 293-Vβ8⁺ cells in triplicate wells at a CAR-T:Lenti-X 293 cell ratio of 2:1 for 24 h (n = 3 biologically independent samples/group). The indicated cytokines in the supernatants were analyzed by performing a CBA assay. The data shown represent the mean ± SEM. Statistical significance was determined by two-sided, two-way ANOVA with Sidaks multiple comparisons test. b–e Untransduced T cells or Vβ8-CAR-T cells were cocultured with Jurkat-Vβ8^- or Jurkat-Vβ8⁺ cells in triplicate wells at a CAR-T: Jurkat cell ratio of 1:2 for 24 h (n = 3 biologically independent samples/group). Cytotoxicity was determined by analyzing the remaining IRFP⁺ cells with flow cytometry (b). The indicated cytokines in the supernatants were analyzed by performing CBA assays (c). Granzyme B (d) and active caspase-3 (e) were analyzed by flow cytometry. The data shown represent the mean ± SEM. Statistical significance was determined by two-sided unpaired t-tests. The normality of the data was confirmed by the Shapiro–Wilk test.