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. 2022 Jul 26;12:12757. doi: 10.1038/s41598-022-16605-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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N-glycans released from extracts of MDCK cells by treatment with PNGase F, permethylated, and analyzed by MALDI-TOF MS. The medial molecular range between m/z 1500 and 5500 is shown. Putative structures based on compositions and corresponding to masses detected are represented next to the corresponding mass peaks. (A) N-glycans from MDCK cells at passage 3, and (B) N-glycans from MDCK cells at passage 23. (C) N-glycans in (A) from extracts of MDCK cells at passage 3 were treated with neuraminidase A and analyzed. (D) N-glycans in (B) from extracts of MDCK cells at passage 23 were treated with neuraminidase A and analyzed. (E) N-glycans in (A) from extracts of MDCK cells at passage 3 were treated with neuraminidase S and analyzed. (F) N-glycans in (B) from extracts of MDCK cells at passage 23 were treated with neuraminidase S and analyzed. The assignments of N-glycan mass and compositions, as well as relative percentage abundance of each glycan species in (A), (C), and (E) are presented in Supplementary Table 3.