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. 2022 Jul 26;12:12757. doi: 10.1038/s41598-022-16605-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Lectin and Western blot analyses of total glycoproteins in MDCK, SIAT1, and hCK cells. Total cell lysates of MDCK, SIAT1, and hCK cell lines were treated with PNGase F (PNGaseF), Neuraminidase A (NeuA), or Neuraminidase S (NeuS), and separated by SDS-PAGE. Total extracts of cells at passage 3 (top) and passage 23 (bottom) (A–F), plus PNGaseF/neuraminidases mixture (Enzyme mix) control were loaded on the gels. (A) Gel stained with Coomassie Brilliant Blue solution. (B) Transferred total glycoproteins analyzed by lectin blot using ConA; (C) SNA; (D) MAL-I; and (E) PHA-E. (F) Total glycoproteins from cells at passage 3, and 23 were Western blotted with the antibody O6.