Fig. 4.

Phylogenetic relationships between the isolates IOC 2547, IOC 1835, IOC 1912, IOC 1799 IOC 1275, IOC 2993 and IOC 2835 with reference strains of the Sporothrix schenckii complex inferred from β-tubulin sequences by Neighbor-Joining method [27]. The optimal tree is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [28]. The evolutionary distances were computed using the Maximum Composite Likelihood method [26] and are in the units of the number of base substitutions per site. This analysis involved 31 nucleotide sequences. There were a total of 428 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [25]. Bootstrap support values above 80% are indicated at the nodes.