Table 2.
Cytotoxicity of EA to MLA cells abrogated by 10 mM GSH
| Ethyl acrylate (µg/ml) | Ethyl acrylate only | Ethyl acrylate + 10 mM glutathione simultaneously | Ethyl acrylate treatment followed by posttreatment with 10 mM glutathione | Ethyl acrylate + 10 µM 2-mercaptoethanol | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RGDT | RSG | RTG | RGDT | RSG | RTG | RGDT | RSG | RTG | RGDT | RSG | RTG | |
| 0 (Vehicle control) | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| 1.0 | 101.0 | 102.2 | 128.7 | 88.6 | 87.3 | 75.6 | 99.9 | 97.9 | 78.6 | 89.5 | 106.9 | 97.5 |
| 5.0 | 82.1 | 79.6 | 112 | 77.5 | 79.5 | 68.8 | 88.3 | 86.0 | 78.0 | 89.3 | 64.9 | 69.2 |
| 10.0 | 74.2 | 65.5 | 68.6 | 87.6 | 81.9 | 68.6 | 86.1 | 76.1 | 71.3 | 86.5 | 87.3 | 87.3 |
| 15.0 | 70.9 | 57.1i | 58.9 | 92.8 | 75.8 | 65.6 | 81.2 | 65.6 | 55.9 | 83.9 | 71.9 | 73.1 |
| 20.0 | 45.7 | 78.0 | 77.5 | 62.5 | ||||||||
| 25.0 | 67.5 | 37.3 | 37.9 | 87.3 | 83.1 | 62.2 | 74.7 | 43.8 | 37.3 | 86.4 | 72.9 | 70.7 |
| 30.0 | 61.5 | 17.5 | 14.2 | 89.5 | 83.2 | 69.7 | 82.3 | 17.2 | 7.5 | 79.3 | 66.5 | 51.7 |
| 35.0 | 59.3 | 6.6 | !1.9 | 95.7 | 86.4 | 76.1 | 80.8 | nc | nc | 76.4 | 41.8 | 42.4 |
RGDT relative growth during treatment [%]; RSG relative suspension growth [%]; RTG relative total growth [%]; n.c. culture not continued due to strong cytotoxicity