Table 5.
No mutagenicity of MA in presence of 1 mM GSH
| Methyl acrylate [µg/ml] | Number of empty wellsa | Mutant frequency [per 106 cells] | Colony counts (%) (Absolute counts) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Plate 1 | Plate 2 | Mean | Corrected MFb | Corrected mean MFb | Small colonies | Large colonies | ||||||
| Vehicle control | A | 80 | 85 | 82.5 | 101.3 | 70.4 | 14 | 8 | 83 | 3 | 3 | 17 |
| B | 83 | 90 | 86.5 | 46.5 | 13 | 6 | (10) | 2 | 0 | (2) | ||
| 5 | A | 71 | 77 | 74.0 | 128.5 | 98.5 | 17 | 10 | 65 | 9 | 11 | 35 |
| B | 86 | 79 | 82.5 | 71.2 | 9 | 15 | (13) | 3 | 3 | (7) | ||
| 10 | A | 73 | 84 | 78.5 | 119.7 | 93.8 | 14 | 14 | 69 | 11 | 1 | 31 |
| B | 85 | 84 | 84.5 | 70.4 | 10 | 6 | (11) | 2 | 6 | (5) | ||
| 15 | A | 88 | 72 | 80.0 | 77.2 | 66.7 | 5 | 12 | 64 | 3 | 13 | 36 |
| B | 89 | 83 | 86.0 | 54.3 | 6 | 14 | (9) | 1 | 1 | (5) | ||
| 20 | A | 77 | 75 | 76.0 | 95.3 | 73.1 | 14 | 11 | 65 | 7 | 11 | 35 |
| B | 84 | 85 | 84.5 | 52.0 | 9 | 11 | (11) | 4 | 1 | (6) | ||
| 25 | A | 84 | 84 | 84.0 | 64.9 | 57.0 | 9 | 10 | 82 | 3 | 2 | 18 |
| B | 84 | 86 | 85.0 | 49.6 | 9 | 9 | (9) | 3 | 1 | (2) | ||
| 30 | A | 85 | 82 | 83.5 | 68.9 | 75.7 | 11 | 14 | 87 | 2 | 1 | 13 |
| B | 84 | 78 | 81.0 | 82.5 | 11 | 16 | (13) | 2 | 4 | (2) | ||
| 35 | A | 86 | 88 | 87.0 | 46.3 | 49.9 | 8 | 7 | 80 | 2 | 1 | 20 |
| B | 84 | 87 | 85.5 | 53.5 | 9 | 9 | (8) | 3 | 0 | (2) | ||
aNumber of wells without colonies 10 days after seeding about 2000 cells/well into selection medium
bMutation frequency corrected on the basis of the absolute cloning efficiency 2 (CE2) at the end of the expression period