Table 8.
Ethyl acrylate initially added [µg/ml] | Glutathione initially added [µM] | Ethyl acrylate finally recovereda [µg/ml]/%c/%d | Glutathione finally recoveredb [µmol/g cell lysate] |
---|---|---|---|
0 | 0 | ˂0.2 | 53.3 |
20 | 0 | 11/55 | 9.28 |
25 | 0 | 13/52 | 8.39 |
35 | 0 | 18/51 | 5.55 |
0 | 10 | ˂0.2 | 56.25 |
20 | 10 | 7.5/37c/68d | 16.51 |
25 | 10 | me | 10.98 |
35 | 10 | 13/37/72 | 6.22 |
0 | 100 | ˂0.2 | 65.02 |
20 | 100 | 5.3/26/48 | 22.83 |
25 | 100 | 6.4/26/49 | 17.30 |
35 | 100 | 8.9/25/49 | 14.48 |
0 | 1000 | ˂0.2 | 48.08 |
20 | 1000 | 0.27/1.3/2.5 | 84.74 |
25 | 1000 | 0.48/1.9/3.7 | 82.12 |
35 | 1000 | 0.73/2.1/4.1 | 82.70 |
aIn the end of the mutagenicity experiment recovered in the culture supernatant (in the culture cell lysate ˂1 µg/ml)
bIn the end of the mutagenicity experiment recovered in the culture cell lysate (in the culture supernatant 0, except after the initial addition of 1000 µM GSH 4.15, 2.05, 2.97 and 3.05 µmol/g cell lysate after co-incubation with 0, 20, 25 and 35 µg ethyl acrylate/ml, respectively
cCompared with the initially added amount
dCompared with the residual amount after the exposure period in absence of GSH
eMistake