Table 3.
Main symptoms elicited in mice after acute i.p. administration of MTX1 and number of animals with symptoms at each MTX1 dose
| I.p. MTX dose (ng/kg) | 200 | 400 | 800 | 1000 | 1200 | 1600 | 3200 |
|---|---|---|---|---|---|---|---|
| Lethargy | 1/3 | 5/5 | 10/10 | 7/7 | 7/7 | 5/5 | 4/4 |
| Piloerection | 1/3 | 2/5 | 7/10 | 7/7 | 7/7 | 5/5 | 4/4 |
| Stretching | 2/3 | 5/5 | 5/10 | 7/7 | 7/7 | 5/5 | 4/4 |
| Straub tail reaction | 0/3 | 2/5 | 5/10 | 5/7 | 7/7 | 5/5 | 4/4 |
| Ataxia | 0/3 | 2/5 | 7/10 | 5/7 | 7/7 | 5/5 | 4/4 |
| Kyphosis | 0/3 | 0/5 | 2/10 | 5/7 | 5/7 | 5/5 | 0/4 |
| Dyspnea | 0/3 | 0/5 | 4/10 | 5/7 | 5/7 | 3/5 | 4/4 |
| Cyanosis | 0/3 | 0/5 | 1/10 | 2/7 | 2/7 | 2/5 | 0/4 |
| Abdominal swelling | 0/3 | 0/5 | 5/10 | 7/7 | 7/7 | 5/5 | 4/4 |