Fig. 8.

LC–MS/MS data for the intracellular concentrations of 11 different BAs in PCLS incubated for 48 h in the presence of the humanized bile acid (BA) mixture and with or without a cholestatic drug: a chlorpromazine (CPZ) (15, 30 µM), b cyclosporin A (CSA) (1, 6 µM), or c glibenclamide (GB) (25,65 µM). Primary BAs are shown in green (CA and CDCA), secondary BAs are shown in blue (UDCA, HDCA, DCA and LCA), taurine-conjugated BAs are shown in yellow (TCA, TCDCA, TDCA and THDCA), and the glycine-conjugated BA is shown in orange (GCA). Data are presented as absolute values (nmol BA per mg protein) ± SEM; individual values can be found in Table 4. Graphs present cumulative values of mean values of individual BAs for 5 independent experiments (n = 5 mice), using three slices per mouse for each condition. One-way ANOVA was performed, followed by Tukey’s post hoc test to compare all means (*p < 0.05; **p < 0.01; ***p < 0.001)